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KMID : 0616120160470030237
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Korean Journal of Pharmacognosy
2016 Volume.47 No. 3 p.237 ~ p.245
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Quantitative Analysis of (+)-Catechin, Paeoniflorin, and Paeonol in Moutan Radicis Cortex and Its Processed Products
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Seo Chang-Seob
Kim Jung-Hoon
Shin Hyeun-Kyoo
Kim Byung-Soo
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Abstract
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In present study, we conducted quantification analysis of phenolic compound (paeonol), monoterpene glycoside (paeoniflorin), and tannin ((+)-catechin in the 70% ethanol extracts of non-processed Moutan Radicis Cortex (MRC) and processed MRC by roasting using a high-performance liquid chromatography coupled with photodiode array detector. Three marker components were separated on Gemini C18 analytical column and the column was maintained at 40°C using two mobile phase system consisting of 1.0% (v/v) aqueous acetic acid and 1.0% (v/v) acetic acid in acetonitrile. The flow rate and injection volume were 1.0 mL/min and 10 mL. In non-processed MRC sample, the concentrations of three marker compounds, (+)-catechin, paeoniflorin, and paeonol were 0.20, 1.18, and 2.12%, respectively. On the other hand, the concentrations of the three compounds in processed MRC samples were 0.03?0.24, not detected?1.08, and 0.76?1.82%, respectively.
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KEYWORD
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Moutan Radicis Cortex, Processing Method, (+)-Catechin, Paeoniflorin, Paeonol
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